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Material

From Genes to Atomic Structures: an Introduction to Synchrotron-Based Structural Biology | (smr 2336)
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  • Monday, 23 April 2012
    • 08:30 - 09:20 Registration 
      All Speakers and Participants are required to complete registration formalities
      Location: Adriatico Guest House (Lower Level 1)
      • 08:30 Registration 50'
    • 09:20 - 09:30 Welcome address
      • 09:20 Welcome address 10'
        Speaker: Joseph Niemela (ICTP, Trieste, Italy)
    • 09:30 - 10:30 An Introduction to Crystallography
      • 09:30 An Introduction to Crystallography 1h0'
        Speaker: Bernhard Rupp (k.k.Hofkristallamt, San Marcos, USA)
        Material: lecture notes
    • 10:30 - 11:20 An Introduction to Crystallization
      • 10:30 An Introduction to Crystallization 50'
        Speaker: Silvia Onesti (Sincrotrone Trieste, Italy)
        Material: lecture notes
    • 11:20 - 11:40 Coffee Break
      • 11:20 Coffee Break 20'
    • 11:40 - 12:10 Random Microseeding
      • 11:40 Random Microseeding 30'
        Speaker: Patrick Shaw Stewart (Douglas Instruments Ltd., U.K.)
        Material: lecture notes
    • 12:10 - 13:00 Cloning, Protein Expression and Purification - part 1
      • 12:10 Cloning, Protein Expression and Purification - part 1 50'
        Speaker: Tamar Unger (Weizmann Institute of Science, Rehovot, Israel)
    • 13:00 - 13:50 Lunch Break
      • 13:00 Lunch Break 50'
    • 13:50 - 14:30 Afternoon lectures at Elettra. 
      A private bus will take participants to Elettra for the afternoon session. All participants are requested to meet near bus no.36 bus stop at 1.50pm sharp.
      • 13:50 Afternoon lectures at Elettra. 40'
    • 14:30 - 16:30 Cloning, Protein Expression and Purification - part 2 
      at Elettra
      • 14:30 Cloning, Protein Expression and Purification - part 2 2h0'
        Speaker: Tamar Unger (Weizmann Institute of Science, Rehovot, Israel)
    • 16:30 - 18:30 Crystallization Practicals 
      at Elettra
      • 16:30 Crystallization Practicals 2h0'
        Speaker: Barbara Medagli / Patrick Shaw Stewart (Sincrotrone Trieste, Italy / Douglas Instruments Ltd., U.K)
    • 19:30 - 21:30 Welcome Reception
      Location: Adriatico Guest House Cafeteria
      • 19:30 Welcome Reception 2h0'
  • Tuesday, 24 April 2012
    • 09:00 - 10:00 An Introduction to Synchrotron Radiation
      Location: Adriatico Guest House Giambiagi Lecture Hall
      • 09:00 An Introduction to Synchrotron Radiation 1h0'
        Speaker: Mohammad Yousef (Southern Illinois University, USA)
        Material: lecture notes
    • 10:00 - 10:45 Introduction to Data Collection. Practical Issues plus an Introduction to the Ewald Sphere and the Reciprocal Lattice
      Location: Adriatico Guest House Giambiagi Lecture Hall
      • 10:00 Introduction to Data Collection. Practical Issues plus an Introduction to the Ewald Sphere and the Reciprocal Lattice 45'
        Speaker: Andrew Leslie (University of Cambridge, U.K.)
        Material: lecture notes
    • 10:45 - 11:05 Coffee Break
      Location: Adriatico Guest House Giambiagi Lecture Hall
      • 10:45 Coffee Break 20'
    • 11:05 - 11:50 Data Processing with Mosflm
      Location: Adriatico Guest House Giambiagi Lecture Hall
      • 11:05 Data Processing with Mosflm 45'
        Speaker: Andrew Leslie (University of Cambridge, U.K.)
        Material: lecture notes
    • 11:50 - 12:45 Scaling and Merging Data
      Location: Adriatico Guest House Giambiagi Lecture Hall
      • 11:50 Scaling and Merging Data 55'
        Speaker: Andrew Leslie (University of Cambridge, U.K.)
        Material: lecture notes
    • 12:45 - 14:30 Lunch Break
      Location: Adriatico Guest House Giambiagi Lecture Hall
      • 12:45 Lunch Break 1h45'
    • 14:30 - 17:30 Data Processing Practicals
      Location: Adriatico Guest House - Eklund Informatics Lab (Lower Level 1)
      • 14:30 Data Processing Practicals 3h0'
        Speaker: A. Leslie / M. Yousef (Southern Illinois University, USA / University of Cambridge, U.K.)
  • Wednesday, 25 April 2012
    • 09:00 - 10:45 Structure Solution (I) Electron Density Recontruction and Molecular Replacement
      Location: Adriatico Guest House Giambiagi Lecture Hall
      • 09:00 Structure Solution (I) Electron Density Recontruction and Molecular Replacement 1h45'
        Speaker: Bernhard Rupp (k.k.Hofkristallamt, San Marcos, USA)
    • 10:45 - 11:15 Coffee Break
      Location: Adriatico Guest House Giambiagi Lecture Hall
      • 10:45 Coffee Break 30'
    • 11:15 - 13:00 Structure Solution (II) Experimental Phasing and Density Modification
      Location: Adriatico Guest House Giambiagi Lecture Hall
      • 11:15 Structure Solution (II) Experimental Phasing and Density Modification 1h45'
        Speaker: Bernhard Rupp (k.k.Hofkristallamt, San Marcos, USA)
    • 13:00 - 14:30 Lunch Break
      Location: Adriatico Guest House Giambiagi Lecture Hall
      • 13:00 Lunch Break 1h30'
    • 14:30 - 16:30 Model Building
      Location: Adriatico Guest House Giambiagi Lecture Hall
      • 14:30 Model Building 2h0'
        Speaker: Anastassis Perrakis (Netherlands Cancer Institute, Amsterdam, the Netherlands)
        Material: lecture notes
  • Thursday, 26 April 2012
    • 09:00 - 11:00 Structure Refinement
      Location: Adriatico Guest House Giambiagi Lecture Hall
      • 09:00 Structure Refinement 2h0'
        Speaker: Roberto Steiner (King's College London, U.K.)
    • 11:00 - 11:30 Coffee Break
      Location: Adriatico Guest House Giambiagi Lecture Hall
      • 11:00 Coffee Break 30'
    • 11:30 - 13:00 Proteopedia
      Location: Adriatico Guest House Giambiagi Lecture Hall
    • 13:00 - 14:30 Lunch Break
      Location: Adriatico Guest House Giambiagi Lecture Hall
      • 13:00 Lunch Break 1h30'
    • 14:30 - 17:30 Computational Crystallography Practicals
      Location: Adriatico Guest House - Eklund Informatics Lab (Lower Level 1)
      • 14:30 Computational Crystallography Practicals 3h0'
        Speaker: Roberto Steiner/Anastassis Perrakis (King's College London, U.K. / Netherlands Cancer Institute, Amsterdam, the Netherland)
  • Friday, 27 April 2012
    • 09:00 - 10:30 A Brief Overview of Single Particle Electron Microscopy
      Location: Adriatico Guest House Giambiagi Lecture Hall
      • 09:00 A Brief Overview of Single Particle Electron Microscopy 1h30'
        Speaker: Silvia Onesti (Sincrotrone Trieste, Italy)
        Material: lecture notes
    • 10:30 - 11:00 Coffee Break
      Location: Adriatico Guest House Giambiagi Lecture Hall
      • 10:30 Coffee Break 30'
    • 11:00 - 12:00 SAXS
      Location: Adriatico Guest House Giambiagi Lecture Hall
      • 11:00 SAXS 1h0'
        Speaker: Heinz Amenitsch (Elettra, Trieste, Italy)
    • 12:00 - 13:00 SAXS: Case Studies
      Location: Adriatico Guest House Giambiagi Lecture Hall
      • 12:00 SAXS: Case Studies 1h0'
        Speaker: Zehra Sayers (Sabanci University, Istanbul, Turkey)
        Material: lecture notes
    • 13:00 - 13:50 Lunch Break
      Location: Adriatico Guest House Giambiagi Lecture Hall
      • 13:00 Lunch Break 50'
    • 13:50 - 14:30 Afternoon lectures at Elettra. 
      A private bus will take participants to Elettra for the afternoon session. All participants are requested to meet near bus no.36 bus stop at 1.50pm sharp.
      Location: Adriatico Guest House Giambiagi Lecture Hall
      • 13:50 Afternoon lectures at Elettra. 40'
    • 14:30 - 16:00 Data Collection at XRD1 
      at Elettra
      Location: Adriatico Guest House Giambiagi Lecture Hall
      • 14:30 Data Collection at XRD1 1h30'
        Speaker: Doriano Lamba/ Mohammad Yousef (Istituto di Cristallografia, Trieste, Italy / Southern Illinois University, USA)
    • 16:00 - 17:30 Visit to the Free Electron Laser Laboratory - FERMI 
      at Elettra
      Location: Adriatico Guest House Giambiagi Lecture Hall
      • 16:00 Visit to the Free Electron Laser Laboratory - FERMI 1h30'
        Speaker: Giorgio Paolucci (Elettra, Trieste, Italy)
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