Scientific Calendar Event



Description
The Abdus Salam International Centre for Theoretical Physics (ICTP, Trieste, Italy), is organizing an
Advanced Workshop on Structural Biology: using Synchrotron Radiation to Visualise Biological Molecules to be held at the ICTP from 15 -19 December 2014. 

The objective of this Workshop is to expose the participants to the methodologies used to solve macromolecular structures, using synchrotron radiation X-ray diffraction and scattering techniques. 
Structural biology is an interdisciplinary research area, requiring expertises from both the life 
sciences and the physical sciences, so it is often difficult to educate and train young scientists with 
the necessary breath of experience. 
The Workshop is meant to provide a comprehensive overview of the state of the art in the fields of macromolecular crystallography and small-angle X-ray scattering, and aims to bridge the gap between physics and biology by covering the entire process, starting from the gene to obtain the atomic structure. A few practical sessions will provide the students with some hands-on experience in some of the subjects covered by the lectures. 

This event is part of a joint initiative between ICTP, IAEA, Elettra and SESAME, the Middle East
Synchrotron Radiation facility in Jordan. 

Topics will include: 

Protein production; 
Crystallization; 
MX data collection and processing; 
MX structure determination; 
Refinement; 
Model building; 

Hands-on sessions will include: 
Crystallization; 
Synchrotron data collection; 
Data processing; 
Refinement & Model building. 
SAXS data collection and structure determination; 
Complementary techniques. 
Go to day
  • Monday, 15 December 2014
    • 08:30 - 09:00
      • 08:30 REGISTRATION & ADMINISTRATIVE FORMALITIES 30'
    • 09:00 - 14:30 Morning Session
      • 09:00 Welcome address 5'
        Speaker: Joseph NIEMELA (ICTP)
      • 09:10 An introduction to crystallography 50'
        Speaker: Bernhard RUPP (k.k. Hofkristallamt, Vista, U.S.A.)
        Material: Slides locked
      • 10:00 Cloning, protein expression and purification - Part 1 1h0'
        Speaker: Tamar UNGER (Weizmann Institute of Science, Rehovot, Israel)
        Material: Slides locked
      • 11:00 Coffee break 20'
      • 11:20 Cloning, protein expression and purification - Part 2 1h40'
        Speaker: Tamar UNGER (Weizmann Institute of Science, Rehovot, Israel)
        Material: Slides locked
      • 13:00 Lunch break 1h30'
    • 14:30 - 18:00 Afternoon Session
      • 14:30 Data collection & Processing 1h30'
        Speaker: Michele CIANCI (EMBL Hamburg, Germany)
        Material: Slides
      • 16:00 Coffee break 30'
      • 16:30 Data collection & processing 1h30'
        Speaker: Michele CIANCI (EMBL Hamburg, Germany)
        Material: Slides
  • Tuesday, 16 December 2014
    • 09:00 - 14:30 Morning Session
      • 09:00 Structure solution - Part 1 1h45'
        Speaker: Bernhard RUPP (k.k. Hofkristallamt, Vista, U.S.A.)
        Material: Slides locked
      • 10:45 Coffee break 30'
      • 11:15 Structure solution - Part 2 1h45'
        Speaker: Bernhard RUPP (k.k. Hofkristallamt, Vista, U.S.A.)
        Material: Slides locked
      • 13:00 Lunch break 1h30'
    • 14:30 - 17:30 Afternoon Session
      • 14:30 Data processing practicals 3h0'
        Speaker: Michele CIANCI (EMBL Hamburg, Germany)
        Material: Slides
  • Wednesday, 17 December 2014
    • 09:00 - 14:00 Morning Session
      • 09:00 Model building 2h0'
        Speaker: Anastassis PERRAKIS (Netherlands Cancer Institute, Amsterdam)
      • 11:00 Coffee break 30'
      • 11:30 Proteopedia 1h30'
        Speaker: Joel SUSSMAN (Weizmann Institute of Science, Rehovot, Israel)
        Material: Slides
      • 13:00 Lunch break 1h0'
    • 14:00 - 18:30 Afternoon Session - Elettra: Transportation will be provided to take all participants to Elettra Sincrotrone Trieste. A bus will be waiting near the seafront (near the no. 6 bus stop) from 14.00 ready to leave at 14.10. Please be there on time.
      • 15:00 Crystallization & Data collection practicals 3h30'
        Speaker: Doriano LAMBA (Istituto di Cristallografia C.N.R., Trieste)
  • Thursday, 18 December 2014
    • 09:00 - 14:30 Morning Session
      • 09:00 Structure refinement 2h0'
        Speaker: Roberto STEINER (King's College London, U.K.)
        Material: Slides
      • 11:00 Coffee break 30'
      • 11:30 AFM techniques & structural biology 1h30'
        Speaker: Loredana CASALIS (Elettra Sincrotone Trieste)
      • 13:00 Lunch break 1h30'
    • 14:30 - 20:30 Afternoon Session
      • 14:30 Computational crystallography practicals 3h0'
        Speaker: STEINER Roberto / PERRAKIS Anastassis (King's College London, U.K. / Netherlands Cancer Institute, Amsterdam)
      • 18:30 Reception 2h0'
  • Friday, 19 December 2014
    • 09:00 - 14:30 Morning Sessin
      • 09:00 Small-angle X-ray scattering 2h0'
        Speaker: Zehra SAYERS (Sabanci University, Istanbul, Turkey)
      • 11:00 Coffee break 30'
      • 11:30 A brief overview of single particle electron microscopy 1h30'
        Speaker: Silvia ONESTI (Elettra Sincrotone Trieste)
      • 13:00 Lunch break 1h30'
    • 14:30 - 17:00 Afternoon Session
      • 14:30 Drug design 1h30'
        Speaker: Menico RIZZI (Universita' degli Studi del Piemonte Orientale Amedeo Avogadro, Novara, Italy)
      • 16:00 Crystallography & bacterial diseases 1h0'
        Speaker: KAPLAN-TURKOZ Burcu (Ege University, Izmir, Turkey)