Winter College on Optics: Advanced Optical Techniques for Bio-imaging

Administration

Description

The College will address fundamental and experimental aspects of advanced Microscopy, Spectroscopy and related techniques. The scope of the course is to promote new theoretical and experimental methods, concepts, instruments, measurement techniques and data analysis routines for both Laboratory and industrial applications to train students and scientists, as well as to coordinate international activities and collaborations in this area. By focusing on both theory and applications, the College will also provide an interesting intersection of emerging techniques and experimental methods with theoretical advances in the field. The lectures will focus on a variety of topics related to biological applications, environmental research and material characterization. Hands-on sessions will be organized every day of the college in the ICTP laboratories accompanied by library documentation finalized with round-table discussion.

MAIN TOPICS

• Introduction to optical microscopy, confocal microscopy, phase contrast microscopy and super-resolution
• Bio-imaging processing and speckle interferometry bio-imaging
• Advanced fluorescence microscopy, fluorescence nanoscopy and label free approaches in microscopy
• Mobile-phone based fluorescent microscopy: sensing and diagnostics
• STED microscopy: the way to Nobel Prize
• Polarization microscopy: biomedical imaging and diagnostics
• Photothermal spectroscopy and microscopy: related techniques and applications
• Time-resolved and multispectral spectroscopy. Biosensing by surface plasmon resonance
• Optical properties of thin films, optical lithography, applications of nanostructured porous materials for biomedicine
• Optical tweezers and applications

PREPARATORY SCHOOL:

An ICTP Preparatory School will be organized the week before the College (from 6 to 10 February 2017) for a limited number of selected participants. The Preparatory School will provide background tutorials and exercises designed to help the participants in following the College lectures.

Additional Information from the Marie Curie Library:
OSA and SPIE granted free access to their full text online databases.
Access will be available between the 1st and the 24th of February from the ICTP intranet: LINK TO THE ONLINE DATABASES
(in case of connection problems please check SETUP REQUIREMENTS)
The Marie Curie Library is available for any further assistance in consulting these or other resources.
http://library.ictp.it/

Material

Final List Participants General Information Photos Poster group photo

Winter College on Optics: Advanced Optical Techniques for Bio-imaging | (smr 3104)

Go to day

Monday, 13 February 2017

08:30 - 18:00 First week

08:30 Registration 2h0' ( Leonardo Building - Lobby )

Only visitors lodging outside ICTP Guesthouses are kindly requested to register from 8:30 to 9:00 at the Leonardo Building – Lobby. The remaining time will be needed to all participants financially supported by ICTP to complete administrative formalities at the Enrico Fermi Building at 8:30.  Please, be all on time to fulfill these formalities. Those who have already registered for the Preparatory School do not need to register again.

10:30 Welcoming and introduction 15' ( Leonardo Building - Budinich Lecture Hall )
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Welcoming speech by Professor Fernando Quevedo, ICTP Director
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10:45 Introduction to optical microscopy: fundamental concepts and developments in modern microscopy 1h45' ( Leonardo Building - Budinich Lecture Hall )

Speaker:	Colin J. Sheppard (Istituto Italiano di Tecnologia, Italy)
Material:	Slides Video

12:30 Lunch break 1h30' ( Leonardo Building - Cafeteria )

14:00 Optical lithography: basics and practice 1h30' ( Leonardo Building - Budinich Lecture Hall )

Speaker:	Nicoleta Tosa (National Institute for Research and Development of Isotopic and Molecular Technologies, Romania)
Material:	Slides Video

15:30 Coffee break 30'

16:00 Experimental sessions: Abbe diffraction experiment 2h0' ( Multidisciplinary Laboratory )

Tutor: Jorge Alex Villabona;    Groups: 4, 5, 6

Speaker:	Humberto Cabrera (Venezuelan Institute for Scientific Research, Venezuela)
Material:	Slides Video

16:00 Experimental sessions: UV-Vis optical fiber assisted spectroscopy in thin films and solutions 2h0' ( Multidisciplinary Laboratory )

Tutor: Zeinab Ebrahimpour;   Groups: 4, 5, 6

Speaker:	Nicoleta Tosa (National Institute for Research and Development of Isotopic and Molecular Technologies, Romania)
Material:	Slides

16:00 Computer lab processing: Low cost microscope automation hardware and embedded software development 2h0' ( Leonardo Building - Computer Lab (Leonardo Building) )
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Tutors: Javier Ramirez and Micaela Toscani;   Groups: 1,2,3
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Material: Slides

Tuesday, 14 February 2017

09:00 - 19:30

09:00 Basic principles of photothermal techniques and their applications 1h30' ( Leonardo Building - Budinich Lecture Hall )

Speaker:	Ernesto Marín Moares (Instituto Politécnico Nacional, Mexico)
Material:	Slides Video

10:30 Coffee break 30'

11:00 Confocal microscopy and super-resolution. Phase contrast microscopy 1h30' ( Leonardo Building - Budinich Lecture Hall )

Speaker:	Colin J. Sheppard (Istituto Italiano di Tecnologia, Italy)
Material:	Slides Video

12:30 Lunch break 1h30' ( Leonardo Building - Cafeteria )

14:00 Experimental sessions: digital lock-in photothermal shadowgraph method 1h30' ( Multidisciplinary Laboratory )

Tutors: Angel Cifuentes and Dorota Korte;    Groups: 1, 2, 3

Speaker:	Humberto Cabrera and Ernesto Marin Moares (Venezuelan Institute for Scientific Research, Venezuela; Instituto Politecnico Nacional, Mexico)
Material:	Video

14:00 Experimental sessions: Laser induced breakdown spectroscopy LIBS 1h30' ( Multidisciplinary Laboratory )

Tutor: Jorge Alex Villabona;    Groups: 1, 2, 3

Speaker:	Luis Ponce (CICATA, Mexico)
Material:	Video

14:00 Computer lab processing: Low cost microscope automation hardware and embedded software development 1h30' ( Adriatico Guest House - Informatics Lab (Lower Level 1) )
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Tutors: Javier Ramirez and Micaela Toscani;   Groups: 4, 5, 6
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Material: Slides
15:30 Coffee break 30'

16:00 Poster session 3h30' ( Leonardo Building - Lobby )

~Refreshments will be served during the poster session~

Wednesday, 15 February 2017

09:00 - 23:30

09:00 Photothermal digital lock-in shadowgraph technique for materials thermal characterization (signal and image digital processing) 1h30' ( Leonardo Building - Budinich Lecture Hall )

Speaker:	Ernesto Marín Moares (Instituto Politécnico Nacional, Mexico)
Material:	Slides Video

10:30 Coffee break 30'

11:00 Thermal lens spectroscopy: principles and applications - part 1 1h30' ( Leonardo Building - Budinich Lecture Hall )

Speaker:	Aristides Marcano (Delaware State University, USA)
Material:	Slides Video

12:30 Lunch break 1h30' ( Leonardo Building - Cafeteria )

14:00 Experimental sessions: laser speckle bio-imaging demonstrations 1h30' ( Multidisciplinary Laboratory )

Tutor: Jorge Alex Villabona;    Groups: 1, 2, 3

Speaker:	Humberto Cabrera and Maria L. Calvo (Venezuelan Institute for Scientific Research, Venezuela; Complutense University of Madrid, Spain)
Material:	Video

14:00 Experimental sessions: UV-Vis optical fiber assisted spectroscopy in thin films and solutions 1h30' ( Multidisciplinary Laboratory )
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Tutor: Zeinab Ebrahimpour;   Groups: 1, 2, 3
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Speaker: Nicoleta Tosa (National Institute for Research and Development of Isotopic and Molecular Technologies, Romania)
14:00 Fluorescent nano-diamond and Arduino programing demonstrations 1h30' ( Adriatico Guest House - Informatics Lab (Lower Level 1) )
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Tutor: Micaela Toscani;   Groups: 4, 5, 6
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15:30 Coffee break 30'

16:00 Experimental sessions: Laser induced breakdown spectroscopy LIBS 2h0' ( Multidisciplinary Laboratory )

Tutor: Jorge Alex Villabona;    Groups: 4, 5, 6

Speaker:	Luis Ponce (CICATA, Mexico)
Material:	Video

16:00 Computer lab processing: shadowgraph method 2h0' ( Leonardo Building - Computer Lab (Leonardo Building) )
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Tutor:  Angel  Cifuentes;   Groups: 1, 2, 3
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Material: Video

16:00 Experimental sessions: digital lock-in photothermal shadowgraph method 2h0' ( Multidisciplinary Laboratory )

Tutors: Angel Cifuentes and Dorota Korte;    Groups: 4, 5, 6

Speaker:	Humberto Cabrera and Ernesto Marin Moares (Venezuelan Institute for Scientific Research, Venezuela; Instituto Politecnico Nacional, Mexico)
Material:	Video

18:00 Experimental sessions: Thermal lens microscopy 2h0' ( Multidisciplinary Laboratory )

Tutors: Muhammad Sulaiman Yousafzai and Dorota Korte;   Groups: 4, 5, 6

Speaker:	Humberto Cabrera and Aristides Marcano (Venezuelan Institute for Scientific Research, Venezuela; Delaware State University, USA)
Material:	Video

Thursday, 16 February 2017

09:00 - 19:00

09:00 Thermal lens spectroscopy: principles and applications – part 2 1h30' ( Leonardo Building - Budinich Lecture Hall )

Speaker:	Aristides Marcano (Delaware State University, USA)
Material:	Slides Video

10:30 Coffee break 30'

11:00 Bio-imaging processing - part 1 1h30' ( Leonardo Building - Budinich Lecture Hall )

Speaker:	Tatiana Alieva (Complutense University of Madrid, Spain)
Material:	Slides Video

12:30 Lunch break 1h30' ( Leonardo Building - Cafeteria )
14:00 Computer lab processing: Low cost actuators and detectors for bio-imaging application experiments 1h30' ( Leonardo Building - Computer Lab (Leonardo Building) )
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Tutors: Jorge Alex Villabona and Micaela Toscani;   Groups: 1,2,3
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14:00 Computer lab processing: Low cost actuators and detectors for bio-imaging application experiments 1h30' ( Adriatico Guest House - Informatics Lab (Lower Level 1) )
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Tutor: Javier Ramirez;   Groups: 4, 5, 6
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Material: Slides
15:30 Coffee break 30'

16:00 LAMP seminars session 1h30' ( Leonardo Building - Budinich Lecture Hall )

Guillermo Alejandro HERNANDEZ MENDOZA (Cellular Physiology Institute, Mexico):
"Instrumentation of an optical nanoscopy to study serotonin release in neurons"

Manmohan SINGH (University of Houston, U.S.A.):
"Characterization of Corneal Biomechanical Properties with Noncontact Optical Coherence Elastography"

Yousef POURVAIS BAHRAMVANDI (University of Zanjan, Iran):
"Quantitative analysis of stainless steel corrosion by reflective digital holographic microscopy"

Suzana TOPUZOSKI (University SS Cyril and Methodius, Macedonia):
"Generation of coupled optical vortices by computer-constructed gratings"

Pezhman SASANPOUR YAZDI (Shahid Beheshti University of Medical Sciences, Iran):
"Nonlinear super resolution fluorescent microscopy with gold nano triangles; a theoretically investigated method"

17:30 Coffee break 30'

18:00 Poster session 1h0' ( Leonardo Building - Lobby )

Refreshments will be served during the poster session

Friday, 17 February 2017

09:00 - 19:00

09:00 Bio-imaging processing - part 2 1h30' ( Leonardo Building - Budinich Lecture Hall )

Speaker:	Tatiana Alieva (Complutense University of Madrid, Spain)
Material:	Slides Video

10:30 Coffee break 30'

11:00 Speckle interferometry: theory and applications 1h0' ( Leonardo Building - Budinich Lecture Hall )

Speaker:	Maria L. Calvo (Complutense University of Madrid, Spain)
Material:	Slides Video

12:00 Lunch break 1h30' ( Leonardo Building - Cafeteria )
13:30 Computer lab processing: shadowgraph method 1h30' ( Adriatico Guest House - Informatics Lab (Lower Level 1) )
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Tutor:  Angel  Cifuentes;   Groups: 4, 5, 6
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13:30 Computer lab processing: Low cost computer data acquisition, device control using microcontrollers and open source software for bio-imaging 1h30' ( Leonardo Building - Computer Lab (Leonardo Building) )
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Tutors: Javier Ramirez, Micaela Toscani and Jorge Alex Villabona;   Groups: 1,2,3
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15:00 Coffee break 30'

15:30 Polarization microscopy: biomedical imaging and diagnostics 1h30' ( Leonardo Building - Budinich Lecture Hall )

Speaker:	Yuriy Ushenko (Chernivtsi National University, Ukraine)
Material:	Slides Video

17:15 Experimental sessions: laser speckle bio-imaging demonstrations 1h45' ( Multidisciplinary Laboratory )

Tutor: Jorge Alex Villabona;    Groups: 4, 5, 6

Speaker:	Humberto Cabrera and M. L. Calvo (Venezuelan Institute for Scientific Research, Venezuela; Complutense University of Madrid, Spain)
Material:	Video

17:15 Experimental sessions: surface plasmon resonance method for precise detection of low concentration solutions 1h45' ( Multidisciplinary Laboratory )

Tutors: Zeinab Ebrahimpour;   Groups: 4, 5, 6

Speaker:	Viktor Lysiuk (V. Lashkariov Institute of Semiconductor Physics, Ukraine)
Material:	Slides

Monday, 20 February 2017

09:00 - 18:00 Second week - Special conference: Professor Rainer Heintzmann on Thursday 23 February 2017

09:00 Advanced fluorescence microscopy: theory and practice 1h30' ( Leonardo Building - Budinich Lecture Hall )

Speaker:	Alberto Diaspro (Istituto Italiano di Tecnologia, Italy)
Material:	Slides Video Video - extra

10:30 Coffee break 30'

11:00 Thermal lens spectrometry and microscopy 1h30' ( Leonardo Building - Budinich Lecture Hall )

Speaker:	Mladen Franko (University of Nova Gorica, Slovenia)
Material:	Slides Video

12:30 Lunch break 1h30' ( Leonardo Building - Cafeteria )

14:00 Mobile-phone based fluorescent microscopy: sensing and diagnostics 1h30' ( Leonardo Building - Budinich Lecture Hall )

Speaker:	Zach Ballard (UCLA, USA)
Material:	Video

15:30 Coffee break 30'

16:00 Experimental sessions: portable mobile phone microscopes, demonstration with different prototypes 2h0' ( Leonardo Building - Budinich Lecture Hall )

Groups: All

Speaker:	Zach Ballard (UCLA, USA)
Material:	Video

Tuesday, 21 February 2017

09:00 - 20:45

09:00 Fluorescence nanoscopy and label free approaches in microscopy (SHG/Mueller matrix) 1h30' ( Leonardo Building - Budinich Lecture Hall )

Speaker:	Alberto Diaspro (Istituto Italiano di Tecnologia, Italy)
Material:	Slides Video Video - extra

10:30 Coffee break 30'

11:00 Applications of thermal lens spectrometry and microscopy 1h30' ( Leonardo Building - Budinich Lecture Hall )

Speaker:	Mladen Franko (University of Nova Gorica, Slovenia)
Material:	Slides Video

12:30 Lunch break 1h30' ( Leonardo Building - Cafeteria )

14:00 Surface plasmon resonance sensing with applications in biological objects and health control 1h0' ( Leonardo Building - Budinich Lecture Hall )

Speaker:	Viktor Lysiuk (V. Lashkariov Institute of Semiconductor Physics, Ukraine)
Material:	Slides Video

15:00 Coffee break 30'

15:30 ICO/ICTP Gallieno Denardo Award Ceremony 1h30' ( Leonardo Building - Budinich Lecture Hall )

Material:

2017 Winner materials

Photos

Video

19:00 ICO Reception 1h45' ( Adriatico Guest House - Cafeteria )

Wednesday, 22 February 2017

09:00 - 18:00

09:00 Laser spectroscopy and multispectral analysis: somme applications 1h30' ( Leonardo Building - Budinich Lecture Hall )

Speaker:	Ahmadou Wagué (University Cheikh Anta Diop, Senegal)
Material:	Slides Video

10:30 Coffee break 30'

11:00 Optical tweezers: basics and applications 1h30' ( Leonardo Building - Budinich Lecture Hall )

Speaker:	Dan Cojoc (National Research Council of Italy, Italy)
Material:	Slides Video

12:30 Lunch break 1h30' ( Leonardo Building - Cafeteria )

14:00 Experimental sessions: optical tweezers, manipulation of micron-size particles and discussion with students 1h30' ( Leonardo Building - Budinich Lecture Hall )

Tutor: Muhammad Sulaiman Yousafzai;   Groups: All

Speaker:	Dan Cojoc (National Research Council of Italy, Italy)
Material:	Slides Video

15:30 Coffee break 30'

16:00 Experimental sessions: surface plasmon resonance method for precise detection of low concentration solutions 2h0' ( Multidisciplinary Laboratory )

Tutor: Jorge Alex Villabona;    Groups: 1, 2, 3

Speaker:	Viktor Lysiuk (V. Lashkariov Institute of Semiconductor Physics, Ukraine)
Material:	Slides

16:00 Experimental sessions: determination of the optical properties of thin films, influence of the substrate and material 2h0' ( Multidisciplinary Laboratory )

Tutor: Zeinab Ebrahimpour;   Groups: 1, 2, 3

Speaker:

Raúl Martín Palma and Nicoleta Tosa (Universidad Autónoma de Madrid, Spain; National Institute for Research and Development of Isotopic and Molecular Technologies, Romania)

16:00 Computer lab processing: Low cost computer data acquisition, device control using microcontrollers and open source software for bio-imaging 2h0' ( Adriatico Guest House - Informatics Lab (Lower Level 1) )
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Tutors: Javier Ramirez and Micaela Toscani;   Groups: 4, 5, 6
```

Thursday, 23 February 2017

09:00 - 18:00

09:00 Computational aspects of SIM -&- Light Wedge and Lightsheet‐Raman Microscopy 1h30' ( Leonardo Building - Budinich Lecture Hall )

Speaker:	Rainer Heintzmann (Leibniz Institute of Photonic Technology, Germany)
Material:	Slides Video

10:30 Coffee break 30'

11:00 Applications of nanostructured porous silicon in biomedicine 1h30' ( Leonardo Building - Budinich Lecture Hall )

Speaker:	Raúl Martín Palma (Universidad Autónoma de Madrid, Spain)
Material:	Slides

12:30 Lunch break 1h30' ( Leonardo Building - Cafeteria )

14:00 Experimental sessions: Demonstration with polarization microscopy, biomedical imaging and diagnostics 1h30' ( Leonardo Building - Budinich Lecture Hall )

Groups: All

Speaker:	Yuriy Ushenko (Chernivtsi National University, Ukraine)
Material:	Slides Video

15:30 Coffee break 30'

16:00 Experimental sessions: determination of the optical properties of thin films, influence of the substrate and material 2h0' ( Multidisciplinary Laboratory )

Tutors: Zeinab Ebrahimpour;   Groups: 4, 5, 6

Speaker:

Raúl Martín Palma and Nicoleta Tosa (Universidad Autónoma de Madrid, Spain; National Institute for Research and Development of Isotopic and Molecular Technologies, Romania)

16:00 Fluorescent nano-diamond and Arduino programing demonstrations 2h0' ( Leonardo Building - Computer Lab (Leonardo Building) )
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Tutor: Micaela Toscani;   Groups: 1,2,3
```

Friday, 24 February 2017

09:00 - 13:00

09:00 Time resolved spectroscopy - part 1 1h30' ( Leonardo Building - Budinich Lecture Hall )

Speaker:	Mikas Vengris (Vilnius University, Lithuania)
Material:	Slides Video

10:30 Coffee break 30'

11:00 Time resolved spectroscopy - part 2 1h30' ( Leonardo Building - Budinich Lecture Hall )

Speaker:	Mikas Vengris (Vilnius University, Lithuania)
Material:	Slides Video

12:30 Feedback Session & Closing Remarks 30' ( Leonardo Building - Budinich Lecture Hall )